JICDRO is a UGC approved journal (Journal no. 63927)
Year : 2011  |  Volume : 3  |  Issue : 1  |  Page : 21-24

An in vitro investigation of a newer intracanal medicament Nisin on Enterococcus faecalis in comparison with chlorhexidine and calcium hydroxide

1 Department of Conservative Dentistry and Endodontics, Indira Gandhi Institute of Dental Sciences, Puducherry, India
2 Department of Conservative Dentistry and Endodontics,Ragas Dental College and Hospital, Chennai, India
3 Department of Conservative Dentistry and Endodontics,Sri Venkateswara Dental College and Hospital, Chennai, India

Correspondence Address:
Suneel Kumar Chinni
Indira Gandhi Institute of Dental Sciences, Pillyarkuppam, Puducherry
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2231-0754.115770

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Aim: To determine the antibacterial efficacy of Nisin in comparison with Calcium hydroxide and Chlorhexidine. Materials and Methods: Fifty extracted lower premolar single-rooted teeth were collected and were instrumented with K3 rotary files. Then, root canals were inoculated with a bacterial solution of Enterococcus faecalis. After 21 days, the canals were inoculated with Saline, Nisin, Vancomycin, Calcium hydroxide, and Chlorhexidine. The roots were left for 7 days and on the 8 th day, to investigate the degree of infection of the radicular dentin, specimens of the dentin chips from the full length of the root canal were harvested using a sterile rotary K3 instrument (size 25 6% taper). Results: The results of the present study showed that Nisin and Chlorhexidine showed none of Colony Forming Units (CFU) in their respective group. Conclusion: Within the limits of the study, Nisin was effective in eradicating E. faecalis cells in pure culture and root canal dentin.

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